Dietary Aloe barbadensis miller supplementation enhances growth performance in Cirrhinus mrigala fingerlings
DOI:
https://doi.org/10.53992/njns.v11i3.350Keywords:
Cirrhinus mrigala, A. barbadensis, WG, FCR, SGRAbstract
Aquaculture faces challenges due to slow growth, high mortality, disease outbreak and poor meat quality. Aloe barbadensis Miller (Aloe vera), a natural and sustainable plant based ingredients due to its immune stimulatory, antioxidant, antibacterial and growth enhancing properties can help to address these issues. This research was conducted to examine the effects of A. barbadensis supplemented feed on growth performance of Cirrhinus mrigala fingerlings. The present research was conducted at Fisheries Research Farm, Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad. Before experiment, fingerlings of Mrigala Carp were acclimatized and then divided into four experimental groups each with three replicates, respectively. The fingerlings were fed 3% of their body weight for 45 days. Four experimental diets were formulated as: T0 (control group), T1 (500mg/kg A. vera extract), T2 (1000mg/kg A. vera extract) and T3 (2000mg/kg A. barbadensis extract. The experimental design of this study was completely randomized design (CRD) using Tukey’s HSD post hoc test for comparing treatment means. The results revealed significant differences between treatments. Growth parameters in terms of body weight (g), body length (cm), SGR (%) and FCR (%) were measured on weekly basis. T1 showed the highest weight gain (1.39g), length gain (1.36cm) and SGR (2.18%) with the lowest FCR (0.90). Maximum survival rate recorded in T1 and T2 (100%) followed by T0 (80%) and T3 (90%). Physico-chemical parameters i.e., temperature, DO and pH were recorded on weekly basis. The results revealed A. vera supplemented feed, particularly 500 mg/kg inclusion, significantly improved growth performance of Cirrhinus mrigala without any adverse effect.
References
rabu E, Felix S, Felix N, Ahilan B, Ruby P. An overview on significance of fish nutrition in aquaculture industry. Int J Fish Aquat Stud. 2017; 5(6):349-355.
Naylor RL, Hardy RW, Buschmann AH, Bush SR, Cao L, Klinger DH, Troell M. A 20-year retrospective review of global aquaculture. Nature. 2021;591(7851):551-563.
Dawood MA, Noreldin AE, Ali MA, Sewilam H. Menthol essential oil is a practical choice for intensifying the production of Nile Tilapia, Effects on the growth and health performances. Aquaculture. 2021;543:737027.
Nousheen S, Amir I, Bukhat H, Farooq U, Batool S. Fish as a functional food: a comprehensive review of bioactive components and their therapeutic potential for human health. Int Aquat Res. 2025;17(3):201-218.
Sikotariya S, Yusufzai SI. Effect of Ocimum sanctum (Tulsi) powder on the growth and survival in Cirrhinus mrigala fingerlings. J Entomol Zool Stud. 2019;7(4):239-244.
Shitote Z, Wakhungu J, China S. Challenges facing fish farming development in western Kenya. Greener J Agric Sci. 2012; 3(5):305-311.
Nousheen S, Amir I, Kazmi A, Islam MF, Ali A, Luna-Arias JP, Pelaez-Acero A. From sea to plate: a review on nutrient composition of fish and its health benefits. Nutrire. 2026;51(1):11.
Kaleem O, Sabi AFBS. Overview of aquaculture systems in Egypt and Nigeria, prospects, potentials and constraints. Aquac Fish. 2021;6(6):535-547.
Idowu TA, Sogbesan OA. Synthetic chemical application in aquaculture production. Int J Environ Agric Sci. 2017;1(1):1-10.
Sharma S, Singh DK, Gurung YB, Shrestha SP, Pantha C. Immunomodulatory effect of stinging nettle (Urtica dioica) and A. barbadensis in broiler chickens. Vet Anim Sci. 2018;6:56-63.
Kuebutornye FK, Abarike ED. The contribution of medicinal plants to Tilapia aquaculture: a review. Aquac Int. 2020;28(3):965-983.
Awad E, Awaad A. Role of medicinal plants on growth performance and immune status in fish. Fish Shellfish Immunol. 2017;67:40-54.
Mahdavi M, Hajimoradloo A, Ghorbani R. Effect of A. barbadensis extract on growth parameters of Common Carp (Cyprinus carpio). World J Med Sci. 2013;9(1):55-60.
Gabriel NN, Qiang J, Ma XY, Xu P, Nakwaya DN. Effects of dietary A. barbadensis crude extracts on digestive enzyme activities and muscle proximate composition of GIFT Tilapia juveniles. S Afr J Anim Sci. 2017;47(6):904-913.
Khan RU, Naz S, De Marzo D, Dimuccio MM, Bozzo G, Tufarelli V, Ragni M. A. barbadensis: a sustainable green alternative to exclude antibiotics in modern poultry production. Antibiotics. 2022;12(1):44-60.
Gabriel NN, Abasubong KP, Maulu S, Rashidian G, Haihambo W. The potential benefits of A. barbadensis products in aqua feed: current knowledge and prospects. Emerg Sustain Aquac Innov Afr. 2023;12:149-166.
Tabassum S, Hussain SM, Ali S, Arsalan M, Ahmad B, Asrar M, Sharif A. Partial replacement of fishmeal with Moringa oleifera leaf meal in practical diets of Cirrhinus mrigala fingerlings. Braz J Biol. 2021;83:e246333.
Nisa S, Fatima M, Shah SZH, Khan N, Aftab B, Ali W, Fatima A. Evaluation of growth, antioxidant status, hepatic enzymes and immunity of nanoselenium-fed Cirrhinus mrigala. PLoS One. 2024;19(9):e0308761.
Muhammad N, Umair M, Khan AM, Yaqoob M, Haider MS, Khan Q, Abbasi AR. Assessment of cultural uses of Mrigal Carp in Gujranwala division, Pakistan. J Wildl Ecol. 2018;2(4):1-9.
Farooq U, Amir I, Bukhat H, Nousheen S, Saleem U. Monitoring temporal changes in Indus River water quality at Chashma Barrage: Implications for Winter Season Ecosystem Health. Int J Environ Sci Tech. 2025;22(3):1455-1468.
Haghighi M, Rohani MS. The effect of powdered ginger (Zingiber officinale) on the haematological and immunological parameters of Rainbow Trout Oncorhynchus mykiss. J Med Plant Herbal Ther Res. 2013;10:8-12.
Batool M, Mahmood M, Ul Ain Q, Ijaz MU, Bano S, Nazir S, Sultan H. Effect of A. barbadensis on growth performance and digestive enzymes activity of Mrigal (Cirrhinus mrigala) against Arsenic sub-lethal toxicity. Pak J Agric Sci. 2024;39(1):61-69.
Kumar S, Prakash C, Chadha NK, Jain KK, Pandey PK. Effect of Dietary A. barbadensis on Growth and Haemato-Immunological Responses of Cirrhinus mrigala fingerlings. Anim Nutr Feed
Technol. 2021;21(3):441-456.
Verma A, Holeyappa SA, Bansal N, Kaur VI. Efficacy of quercetin in ameliorating hypoxia-induced hematological and histopathological alterations in rohu Labeo rohita. Fish Physiol Biochem. 2024;50(3):1171-1187.
Zoral MA. Medicinal plants: are they safe enough for fish health. Aquac Int. 2023;31(2):1077-1096.
Malik MA, Sardar P, Munilkumar S, Varghese T, Kumar S, Gupta G, Bhat NM. Comparative evaluation of raw and fermented Eichhornia crassipes leaf meal in the diet of Cyprinus carpio fingerlings: Effects on growth performance, body composition and haemato-biochemical responses. Anim Feed Sci Technol. 2024;310:115947.
Rahman MH, Alam MA, Flura MM, Mely SS, Sultana S, Islam MR. A Review on the Current Status and Emerging Trends in the Utilization of Fish Feed Additives for Sustainable Production of Oreochromis niloticus. Am J Aquac Anim Sci. 2023;2(1):26-37.
Stavrakidis-Zachou O, Eding EH, Papandroulakis N, Lika K. A nutritional bioenergetic model for farmed fish: effects of food composition on growth, oxygen consumption and waste production. Aquac Nutr. 2025;2025:9010939.
Inzaghi E, Pampanini V, Deodati A, Cianfarani S. The effects of nutrition on linear growth. Nutrients. 2022;14(9): 1752.
Khajeeyan R, Salehi A, Movahhedi Dehnavi M, Hamidian M, Hazrati S. Evaluation of the benefits of plant growth-promoting rhizobacteria and mycorrhizal fungi on biochemical and morphophysiological traits of Aloe barbadensis Mill under water deficit stress. Sci Rep. 2024;14(1):14480.



