Green synthesis of iron nanoparticles using Agaricus bisporus: Evaluation of antifungal and antidiabetic potential
DOI:
https://doi.org/10.53992/njns.v11i1.306Keywords:
Green Synthesis, Mycosynthesis, Agaricus Bisporus, Aspergillus Terreus, Iron Nanoparticles, Antifungal, Antidiabetic, Alpha-Amylase Inhibitory AssaysAbstract
This study explores the green synthesis of iron nanoparticles (FeNPs) using aqueous extracts of Agaricus bisporus (stipe and pileus) and evaluates their antifungal and antidiabetic potential. FeNPs were synthesized at room temperature and under heating conditions, with characterization performed using UV-visible spectroscopy, FTIR, SEM-EDS, and DLS. The formation of FeNPs was confirmed by a color shift to amber and a characteristic absorption peak at 450 nm. FTIR analysis revealed functional groups such as O-H, C=O, and Fe-O, indicating the involvement of mushroom phytochemicals in nanoparticle stabilization. SEM confirmed uniform morphology, with smaller FeNPs (35 nm) achieved using pileus extract (PE) under heating. Antifungal activity against Aspergillus terreus and Aspergillus niger was demonstrated via agar well diffusion assays, with inhibition zones up to 34 mm. FeNPs also exhibited significant antidiabetic potential, with glucose adsorption capacities up to 69% and α-amylase inhibition up to 77%, particularly for smaller nanoparticles. The study highlights the dual functionality of A. bisporus-derived FeNPs, showcasing their promise as sustainable nanomaterials for biomedical applications.
References
Zhang XF, Liu ZG, Shen W, Gurunathan S. Nanoparticles in medicine: Therapeutic applications and developments. Clin Pharmacol Ther. 2016;99(3):252-265.
Wu W, He Q, Jiang C. Magnetic iron oxide nanoparticles: Synthesis and surface functionalization strategies. Nanoscale Res Lett. 2008;3(11):397-415.
Mohanpuria P, Rana NK, Yadav SK. Biosynthesis of nanoparticles: Technological concepts and future applications. J Nanopart Res. 2008;10(3):507-517.
Sharma VK, Sayes CM, Guo B, Pillai SD, Parsons JG, Hwang HM, Kuo GD. Nanomaterials in the environment: Behavior, fate, and remediation. Environ Sci Technol. 2019;53(10): 5583-5594.
Lu AH, Salabas EL, Schüth F. Magnetic nanoparticles: Synthesis, protection, functionalization, and application. Angew Chem Int Ed Engl. 2007;46(8):1222-1244.
Tartaj P, Morales MP, Veintemillas-Verdaguer S, Gonzalez-Carreño T, Serna CJ. The preparation of magnetic nanoparticles for applications in biomedicine. J Phys D Appl Phys. 2003;36(13):R182-R197.
Kumar B, Smita K, Cumbal L, Debut A. Green synthesis of iron nanoparticles using plant extracts: A review. Environ Chem Lett. 2020;18(5):1529-1548.
Iravani S. Green synthesis of metal nanoparticles using plants. Green Chem. 2011;13(10):2638-2650.
Mittal AK, Chisti Y, Banerjee UC. Biosynthesis of silver nanoparticles: Synthesis, mechanism, and medical applications. Adv Colloid Interface Sci. 2013;197-198:1-17.
Rai M, Yadav A, Gade A. Fusarium oxysporum-mediated biosynthesis of silver nanoparticles and their antibacterial activity. Nanomedicine
(Lond). 2009;4(4):399-408.
Vigneshwaran N, Kathe AA, Varadarajan PV, Nachane RP, Balasubramanya RH. Biological synthesis of silver nanoparticles using the fungus Aspergillus flavus. Mater Lett. 2007;61(6):1413-1418.
Philip D. Biosynthesis of Au, Ag and Au–Ag nanoparticles using edible mushroom extract. Spectrochim Acta A Mol Biomol Spectrosc. 2009;73(2): 374-381.
Cheung PCK. Mini-review on edible mushrooms as source of dietary fiber: Preparation and health benefits. Food Chem. 2014;148:1-6.
Valverde ME, Hernández-Pérez T, Paredes-López O. Edible mushrooms: Improving human health and promoting quality life. Int J Microbiol. 2015; 2015:376387.
Sen IK, Maity K, Islam SS. Green synthesis of silver nanoparticles using Agaricus bisporus extract and their antibacterial activity. Int J Biol Macromol. 2013;62:439-445.
Mulvaney P. Surface plasmon spectroscopy of nanosized metal particles. Langmuir. 1996;12(3):788-800.
Manikandan G, Ramasubbu R. Biosynthesis of iron nanoparticles from Pleurotus florida and its antimicrobial activity against selected human pathogens. Int J Pharm Sci Res. 2021;12(1):475-481.
Batool F, Khurshid M, Kanwal Q, Shahzad K, Maqbool S, Shahid M, Akram W, Fatima N, Al-Qahtani WH, Al-Mutairi K. Biologically synthesized iron nanoparticles (FeNPs) from Phoenix dactylifera have anti-bacterial activities. Sci Rep. 2021;11(1):22132.
Salah Ud Din SHK, Haq S, Ahmad P, Khandaker MU, Faruque MRI, Idris AM, Sayyed MI. Bio-synthesized tin oxide nanoparticles: Structural, optical, and biological studies. Crystals. 2022;12(5):614.
Senanayake IC, Rathnayake AR,
Sandamali D, Calabon M, Gentekaki E, Lee HB, Pem D, Dissanayake L, Wijesinghe SN, Bundhun D, Nguyen TTT, Goonasekara ID, Abeywickrama PD, Jayawardena RS, Wanasinghe DN, Jeewon R, Bhat DJ, Xiang MM, Bhunjun CS, Hurdeal VG. Morphological approaches in studying fungi: collection, examination, isolation, sporulation and preservation. Mycosphere. 2020;11(1):2678-2754.
Elsayed EA, El Enshasy H, Wadaan MAM, Aziz R. Mushrooms: A potential natural source of anti-inflammatory compounds for medical applications. Mediators Inflamm. 2014;2014: 805841.
Rehman G, Hamayun M, Iqbal A, Khan SA, Khan H, Shehzad A, Khan AL, Anwar S, Islam B, Lee IJ. Green synthesis and characterization of silver nanoparticles using Azadirachta indica seeds extract: In vitro and in vivo evaluation of antidiabetic activity. Molecules. 2021;26(12):3687.
Haleemkhan A, Naseem B, Vardhini B. Synthesis of nanoparticles from plant extracts. Int J Multidiscip Curr Acad Res. 2015;2:195-203.
Saranya S, Vijayarani K, Pavithra S. Green synthesis of iron nanoparticles using aqueous extract of Musa ornata flower sheath against pathogenic bacteria. Int J Pharm Sci Res. 2017;8(5):2241-2245.
Kalaiarasi R, Jayallakshmi N, Venkatachalam P. Phytosynthesis of nanoparticles and its applications. Plant Cell Biotechnol Mol Biol. 2010;11:1-16.
Ijaz M, Zafar MS, Al-Zereini WA, Al-Azawi AK, Al-Rawi M, Al-Hamadani H, Al-Ashekh M. Synthesis, characterization, and applications of iron oxide nanoparticles. Int J Health Sci. 2023;17(1):64-69.
Kumar V, Thakur M, Sharma P, Goutam SK, Singh S. Green synthesis of iron nanoparticles: Sources and multifarious biotechnological applications. Int J Biol Macromol. 2023; 253(Pt 5):127017.
Okka EZ, Ozturk S, Goksen G. Green synthesis and the formation kinetics of silver nanoparticles in aqueous Inula Viscosa extract. J Mol Liq. 2023;382: 121876.
Liang Y, Jiang L, Xu S, He S, Li J. Synthesis and characterization of Fe3O4 nanoparticles prepared by solvothermal method. J Mater Eng Perform. 2023;32:6465-6473.
Dhanasekaran D, Thajuddin N, Panneerselvam A. Extracellular biosynthesis, characterisation and in-vitro antibacterial potential of silver nanoparticles using Agaricus bisporus. J Exp Nanosci. 2013;8(4):579-588.
Rothen-Rutishauser B, Kuhn DA, Ali Z, Gasser M, Amin F, Parak WJ, Clift MJ. Nanoparticle-cell interactions: overview of uptake, intracellular fate and induction of cell responses. In: Gehr P, Mühlfeld C, Rothen-Rutishauser B, Blank F, editors. Nanoparticles in the Lung. Boca Raton: CRC Press; 2014. p. 153-170.
Xu W, Yang T, Li S, Du L, Chen Q, Li X, Dong J, Zhang Z, Lu S, Gong Y, Zhou L, Liu Y, Tan X. Insights into the synthesis, types and application of iron nanoparticles: The overlooked significance of environmental effects. Environ Int. 2022;158:106980.
Alahmad S, Alrebdi TA, Alshammari MT, Labban N. Nanofabrication and characterization of green-emitting N-doped carbon dots derived from pulp-free lemon juice extract. Opt Mater. 2023;139:113766.
Zhang Y, He X, Li Z, Zhang J, Luo Y, Li H. Bandgap engineering of two-dimensional perovskite semiconductors for high-performance solar cells. J Phys Chem C. 2018;122(17):9308-9317.
Chen Y, Fan Z, Luo Z, Liu X, Lai Z, Li B, Zong Y, Gu L, Zhang H. High-quality and water-resistant perovskite films for optoelectronic applications via a facile post-treatment process.
Nanoscale. 2019;11(13):6235-6242.
Wang Y, Zhang Y, Zhang P, Guo W. High-performance perovskite solar cells with enhanced stability via a facile interfacial passivation strategy. Sci Rep. 2020;10(1):12478.
Singh V, Haque S, Niwas R, Srivastava A, Pasupuleti M, Tripathi CKM. Strategies for fermentation medium optimization: An in-depth review. Biotechnol Adv. 2021;47:107731.
Patel SK, Kalia VC, Lee JK. Integrating strategies for sustainable conversion of waste biomass into dark-fermentative hydrogen and value-added products. ACS Omega. 2022;7(1):24-39.
Xu W, Yang T, Li S, Du L, Chen Q, Li X, Dong J, Zhang Z, Lu S, Gong Y, Zhou L, Liu Y, Tan X. Insights into the synthesis, types and application of iron nanoparticles: The overlooked significance of environmental effects. Environ Int. 2022;158:106980.
Poulsen PB, Buchholz K. History of enzymology with emphasis on food production. In: Whitaker JR, Voragen AGJ, Wong DWS, editors. Handbook of Food Enzymology. New York: Marcel Dekker; 2003. p. 11-20.
Sales PM, Souza PM, Simeoni LA, Silveira D. α-Amylase inhibitors: a review of raw material and isolated compounds from plant source. J Pharm Pharm Sci. 2012;15(1):141-183.
Tan Y, Li S, Li C, Liu S. Glucose adsorption and α-amylase activity inhibition mechanism of insoluble dietary fiber: Comparison of structural and microrheological properties of three different modified coconut residue fibers. Food Chem. 2023;418:135970.
Bamburowicz-Klimkowska M, Kasprzak A, Bystrzejewski M, Grudzinski IP. Characteristics of glucose oxidase immobilized on carbon-encapsulated iron nanoparticles decorated with polyethyleneimine. Polym Bull. 2023;80:1565-1586.
Saif S, Tahir A, Chen Y. Green
synthesis of iron nanoparticles and their environmental applications and implications. Nanomaterials (Basel). 2016;6(11):209.
Ahmad H, Rajagopal K, Shah AH, Bhat AH, Venugopal K. Study of bio-fabrication of iron nanoparticles and their fungicidal property against
phytopathogens of apple orchards. IET Nanobiotechnol. 2017;11(3):230-235.
Khezerlou A, Alizadeh-Sani M, Azizi-Lalabadi M, Ehsani A. Nanoparticles and their antimicrobial properties against pathogens including bacteria, fungi, parasites and viruses. Microb Pathog. 2018;123:505-526.
